2G4B
Structure of U2AF65 variant with polyuridine tract
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 129.388, 129.388, 62.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.262 |
| Rwork | 0.261 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2FZR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11182 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | .6 M ammonium sulfate, 100 mM 2-(N-morpholino) ethanesulfonic acid, sodium salt (MES) pH 6.5, 10% dioxane, 200 mM non-detergent sulfobetaine (NDSB) 195 , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 10 | 1 | 2 | dioxane | ||
| 11 | 1 | 2 | NDSB | ||
| 2 | 1 | 1 | 2-(N-morpholino) ethanesulfonic acid | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | dioxane | ||
| 5 | 1 | 1 | NDSB | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | 2-(N-morpholino) ethanesulfonic acid | ||
| 9 | 1 | 2 | MES |
