2G48
crystal structure of human insulin-degrading enzyme in complex with amylin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 65 |
Unit cell lengths | 262.281, 262.281, 91.106 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.770 - 2.600 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 108791 | |
<I/σ(I)> | 18.9 | 5.2 |
Completeness [%] | 99.0 | 98.9 |
Redundancy | 5.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEGMME5000, Dioxane, Tacsimate, HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |