2G40

Crystal structure of a duf162 family protein (dr_1909) from deinococcus radiodurans at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-26
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	53.410, 53.410, 118.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.280 - 1.700
R-factor	0.18
Rwork	0.178
R-free	0.22000
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.344
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.280		1.760
High resolution limit [Å]	1.700	2.900	1.700
Rmerge	0.067	0.057	0.498
Number of reflections	19674	3636	3373
<I/σ(I)>	18.38	37.2	2.8
Completeness [%]	98.3	99.9	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	8.3	277	20.0% PEG-3350, 0.2M K3Citrate, No Buffer, pH 8.3, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K
2	VAPOR DIFFUSION, SITTING DROP, NANODROP	7.5	277	0.2M MgCl2, 30.0% PEG-400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K