2G3Y

Crystal structure of the human small GTPase GEM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-24
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	51.242, 51.242, 173.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.22039
Rwork	0.218
R-free	0.26925
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rap 1kao
RMSD bond length	0.011
RMSD bond angle	1.318
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.250	2.250
Number of reflections	11803
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	PEG3350, Ammonium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K