2G3S
RNA structure containing GU base pairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2000-11-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.332, 43.998, 54.971 |
| Unit cell angles | 112.10, 99.03, 89.96 |
Refinement procedure
| Resolution | 19.950 - 1.499 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 1.510 |
| High resolution limit [Å] | 1.499 | 1.500 |
| Number of reflections | 30400 | |
| Completeness [%] | 96.2 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M magnesium acetate, 0.1M sodium cacodylate, pH 6.5, 10% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | magnesium acetate | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | magnesium acetate | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | H2O |
