2G3P

STRUCTURE OF THE N-TERMINAL TWO DOMAINS OF THE INFECTIVITY PROTEIN G3P OF FILAMENTOUS PHAGE FD

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1998-02
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	125.220, 78.250, 62.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.000 - 1.900
Rwork	0.260
R-free	0.29900 *
Structure solution method	MIRAS
RMSD bond length	0.006 *
RMSD bond angle	1.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.800	1.850
High resolution limit [Å]	1.800 *	1.790
Rmerge	0.079 *	0.450
Total number of observations	343352 *
Number of reflections	58927 *
<I/σ(I)>	4.7	1.6
Completeness [%]	99.8 *	99.8
Redundancy	5.6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2	21 *	pH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	sodium formate	4.3 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)