2G3M
Crystal structure of the Sulfolobus solfataricus alpha-glucosidase MalA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.170, 173.560, 154.080 |
| Unit cell angles | 90.00, 108.00, 90.00 |
Refinement procedure
| Resolution | 34.250 - 2.550 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Trimmed polyalanine version of PDB CODE 1XSI (A chain) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.087 | 0.304 |
| Number of reflections | 167412 | |
| <I/σ(I)> | 16.78 | 7.1 |
| Completeness [%] | 99.2 | |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 298 | 11% PEG 4000, 0.2 M sodium citrate, 0.1 M sodium acetate, microseeding, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
