2G3K

Crystal structure of the C-terminal domain of Vps28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-10
Detector	MARRESEARCH
Wavelength(s)	0.97797
Spacegroup name	P 61 2 2
Unit cell lengths	117.579, 117.579, 294.116
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.940 - 3.050
R-factor	0.21599
Rwork	0.213
R-free	0.27269
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.749
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.210
High resolution limit [Å]	3.050	3.050
Number of reflections	23615
Completeness [%]	99.5	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1 M AS 100 mM sodium acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1 M AS 100 mM sodium acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1 M AS 100 mM sodium acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K