2G3I
Structure of S.olivaceoviridis xylanase Q88A/R275A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 63 |
| Unit cell lengths | 119.317, 119.317, 54.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.880 - 2.100 |
| R-factor | 0.156 |
| Rwork | 0.155 |
| R-free | 0.19200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | same protein in different crystal form |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.338 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 25881 | |
| <I/σ(I)> | 13.3 | 4.2 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 8.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 2.1M ammonium dehydrogen phosphate, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
