2G3H
Cyanide Binding and Heme Cavity Conformational Transitions in Drosophila melanogaster Hexa-coordinate Hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.314, 33.712, 56.059 |
| Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.15568 |
| Rwork | 0.154 |
| R-free | 0.18776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.530 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 32118 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 10 | 277 | 34% PEG 4K, 0.2M magnesium chloride, 0.05M CAPS, pH 10, VAPOR DIFFUSION, temperature 277K |
