2G37
Structure of Thermus thermophilus L-proline dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-01-23 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.067, 89.590, 94.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.160 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.542 |
| Data scaling software | d*TREK (9.2LDz) |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.399 |
| Total number of observations | 12732 | |
| Number of reflections | 46775 | 4045 |
| <I/σ(I)> | 13.6 | 3 |
| Completeness [%] | 98.0 | |
| Redundancy | 6.24 | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 100 mM MgCl2, 100 mM imidazole pH 7.0, 16% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
