2G34
Human hepatitis B virus T=4 capsid strain adyw complexed with assembly effector HAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 528.530, 366.470, 540.070 |
| Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
| Resolution | 25.031 - 5.053 |
| Rwork | 0.365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 2G33 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 5.090 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Rmerge | 0.095 | 0.660 |
| Number of reflections | 407824 | 17315 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 96.7 | 87.5 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 300 | PEG 5000 MME, butanediol, KCl, NaCl, bicarbonate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
