2G2N
Crystal Structure of E.coli transthyretin-related protein with bound Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.970, 92.670, 58.050 |
| Unit cell angles | 90.00, 103.86, 90.00 |
Refinement procedure
| Resolution | 17.050 - 1.650 |
| R-factor | 0.20017 |
| Rwork | 0.198 |
| R-free | 0.23424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.226 |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.050 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | 0.604 |
| Number of reflections | 44377 | |
| <I/σ(I)> | 22.6 | 2.4 |
| Completeness [%] | 80.0 | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG 550 MME, 100mM MES, 10mM ZnSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
