2G29
crystal structure of the periplasmic nitrate-binding protein NrtA from Synechocystis PCC 6803
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-11-03 |
Detector | SBC-3 |
Wavelength(s) | 0.97899, 0.97915, 0.97132 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.959, 53.800, 65.877 |
Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.500 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.22200 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.668 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.560 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 57966 | |
<I/σ(I)> | 20.3 | 6 |
Completeness [%] | 98.4 | 99.4 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M succinate, 20 mM sodium nitrate, 2.0-2.2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |