2G1A
Crystal structure of the complex between Apha class B acid phosphatase/phosphotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.914, 66.287, 91.841 |
| Unit cell angles | 90.00, 121.06, 90.00 |
Refinement procedure
| Resolution | 50.700 - 2.000 |
| R-factor | 0.16339 |
| Rwork | 0.160 |
| R-free | 0.20115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1N8N |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.398 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.036 |
| Number of reflections | 30906 | |
| Completeness [%] | 96.4 | 79.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | Drop: aphA 6mg/ml, MG2+ 10mM, PMEA 10mM. Reservoir solution: 50mM Na acetate, 0.6% (w/v) Spermine tetrahydrochloride, 22% PEG6000, pH7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
