2G18
Crystal Structure of Nostoc sp. 7120 phycocyanobilin:ferredoxin oxidoreductase (PcyA) Apoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.501, 141.332, 121.842 |
| Unit cell angles | 90.00, 91.29, 90.00 |
Refinement procedure
| Resolution | 43.940 - 2.500 |
| R-factor | 0.21326 |
| Rwork | 0.211 |
| R-free | 0.25716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.501 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.383 |
| Number of reflections | 91664 | |
| <I/σ(I)> | 13.9 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 18% PEG-8000, 25mM MES-NaOH, 50mM Calcium acetate, 10% dioxine, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.50 |
