2G15
Structural Characterization of autoinhibited c-Met kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0037 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 103.803, 103.803, 103.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.23419 |
| Rwork | 0.223 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.097 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 19665 | |
| <I/σ(I)> | 5.3 | 0.7 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 1 M diammonium hydrogen phosphate, 0.2 M Sodium chloride, 0.1 M citrate(pH 5.0), and 7.5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
