2G0C
Structure of the RNA binding domain (residues 404-479) of the Bacillus subtilis YxiN protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97858, 0.97910, 0.94643 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 51.010, 51.010, 64.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.253 |
Rwork | 0.253 |
R-free | 0.27600 |
Starting model (for MR) | de novo |
RMSD bond length | 0.012 |
RMSD bond angle | 1.450 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.026 | 0.323 |
Number of reflections | 9790 | 941 |
<I/σ(I)> | 20.3 | 2.8 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 3.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 291 | precipitant of 3.2-3.6 M (NH4)2SO4 buffered with 0.1 M glycine, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 291 | precipitant of 3.2-3.6 M (NH4)2SO4 buffered with 0.1 M glycine, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 291 | precipitant of 3.2-3.6 M (NH4)2SO4 buffered with 0.1 M glycine, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |