2G03
Structure of a putative cell filamentation protein from Neisseria meningitidis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-08 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 148.687, 148.687, 75.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.18542 |
| Rwork | 0.185 |
| R-free | 0.19847 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.426 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 24065 | |
| Completeness [%] | 93.9 | 78.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 10% 2-propanol, 0.1M MES pH 6.0, 0.2M Calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
