2FZT

CRYSTAL STRUCTURE OF a putative nucleic acid binding protein (TM0693) FROM THERMOTOGA MARITIMA MSB8 AT 2.05 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9797, 0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	49.630, 27.430, 65.380
Unit cell angles	90.00, 102.60, 90.00

Refinement procedure

Resolution	21.700 - 2.050
R-factor	0.197
Rwork	0.194
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.226
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.100
High resolution limit [Å]	2.050	4.940	2.050
Rmerge	0.098	0.047	0.514
Number of reflections	11175	858	809
<I/σ(I)>	8.5
Completeness [%]	99.9		100
Redundancy	3.6	3.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	10.0% iso-Propanol, 20.0% PEG-4000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K