2FZF
Hypothetical Protein Pfu-1136390-001 From Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.865, 71.768, 82.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.700 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29900 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | 3DSCALE |
| Phasing software | SGXPRO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.810 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.062 | 0.183 |
| Number of reflections | 10270 | |
| <I/σ(I)> | 19.3 | 3.1 |
| Completeness [%] | 92.0 | 68.6 |
| Redundancy | 6.34 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 1600mM K2HPO4, 400mM NaH2PO4, 200mM NaCl, pH 8.0, temperature 291K, VAPOR DIFFUSION, SITTING DROP |
