2FZ6
Crystal structure of hydrophobin HFBI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84300 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.900, 49.600, 85.800 |
| Unit cell angles | 90.00, 129.40, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.2239 |
| R-free | 0.27560 |
| Structure solution method | MIR |
| Starting model (for MR) | 1r2m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.021 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.046 | 0.275 |
| Number of reflections | 20790 | |
| <I/σ(I)> | 22.8 | 6.42 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M zinc sulphate, 0.1M sodium cacodylate pH6.5, 9mM OSG, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
