2FZ4

Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9800
Spacegroup name	I 2 3
Unit cell lengths	117.600, 117.600, 117.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.400
Rwork	0.243
R-free	0.26460
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.061
Number of reflections	10811
<I/σ(I)>	40.9	6.6
Completeness [%]	100.0	100
Redundancy	19.9	17.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.2	298	100 mM sodium citrate, 1.0 M LiCl., pH 5.2, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	5.2	298	100 mM sodium citrate, 1.0 M LiCl., pH 5.2, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	5.2	298	100 mM sodium citrate, 1.0 M LiCl., pH 5.2, VAPOR DIFFUSION, temperature 298K