2FYZ

Structural of Mumps virus fusion protein core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	161.171, 60.813, 40.147
Unit cell angles	90.00, 98.36, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.24
Rwork	0.231
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svf
RMSD bond length	0.006
RMSD bond angle	0.946
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	17183

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	15% PEG 800, 0.5M Lithium Sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K