2FYW
Crystal Structure of a Conserved Protein of Unknown Function from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.481, 167.016, 122.011 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.20309 |
| Rwork | 0.200 |
| R-free | 0.25901 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.666 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.118 | 0.740 |
| Number of reflections | 38726 | |
| <I/σ(I)> | 17.9 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 10 % PEG P400, 0.1 M Na acetate, 0.2 M Calcium acetate , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
