2FYO

Crystal structure of rat carnitine palmitoyltransferase 2 in space group P43212

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-09
Detector	MARRESEARCH
Wavelength(s)	0.97853
Spacegroup name	P 43 21 2
Unit cell lengths	67.550, 67.550, 307.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.000 - 2.000
R-factor	0.18212
Rwork	0.179
R-free	0.23457
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.161
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.031	0.074
Number of reflections	49466
<I/σ(I)>	28.04	14.8
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.15 M DL-malic acid 20 % (w/v) PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K