2FYM

Crystal structure of E. coli enolase complexed with the minimal binding segment of RNase E.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	77.054, 124.201, 96.076
Unit cell angles	90.00, 90.58, 90.00

Refinement procedure

Resolution	19.990 - 1.600
R-factor	0.16652
Rwork	0.165
R-free	0.20102
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Enolase structure 1E9I
RMSD bond length	0.009
RMSD bond angle	1.157
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.034	0.253
Number of reflections	216432
<I/σ(I)>	13.7	2.45
Completeness [%]	91.1	94.3
Redundancy	10.3	16.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K