2FY8
Crystal structure of MthK rck domain in its ligand-free gating-ring form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 174.141, 180.965, 252.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.190 - 2.790 |
| R-factor | 0.21286 |
| Rwork | 0.212 |
| R-free | 0.25408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LNQ (MONOMER) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.099 | 0.824 |
| Number of reflections | 95903 | |
| <I/σ(I)> | 20.9 | 1.3 |
| Completeness [%] | 97.2 | 75.5 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.6 M Na/K PO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
