2FXQ

Single-stranded DNA-binding protein from Thermus aquaticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-07
Detector	MARRESEARCH
Wavelength(s)	1.039
Spacegroup name	C 2 2 21
Unit cell lengths	51.140, 163.770, 60.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.19207
Rwork	0.189
R-free	0.24731
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.934
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.050	0.832
Number of reflections	22066
<I/σ(I)>	33.9	2.4
Completeness [%]	100.0	100
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	protein solution : 25mg/ml, 1M NaCl, 10mM Tris pH 7.9. well solution : 25% MPD, 20 mM CaCl2, 0.1 M acetate buffer pH 4.6 mix 1:1, VAPOR DIFFUSION, HANGING DROP, temperature 293K