2FX0
Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792,0.9794,0.9184 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 124.208, 124.208, 79.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.680 - 2.400 |
| R-factor | 0.22335 |
| Rwork | 0.221 |
| R-free | 0.27648 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.172 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.063 | 0.340 |
| Number of reflections | 14561 | |
| <I/σ(I)> | 19.9 | 5 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 40% saturation ammonium sulfate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
