2FW2
Catalytic domain of CDY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.454, 133.579, 82.328 |
| Unit cell angles | 90.00, 117.56, 90.00 |
Refinement procedure
| Resolution | 72.930 - 2.200 |
| R-factor | 0.18833 |
| Rwork | 0.185 |
| R-free | 0.25285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | :2FMB |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.406 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.930 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 73770 | |
| Completeness [%] | 99.4 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 10% PEG4000, 0.1M NaAc pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
