2FVP
A Structural Study of the CA Dinucleotide Step in the Integrase Processing Site of Moloney Murine Leukemia Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-06 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.720, 146.126, 46.863 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.250 |
R-factor | 0.2876 |
Rwork | 0.224 |
R-free | 0.28760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d1u |
RMSD bond length | 0.007 |
RMSD bond angle | 1.373 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 18291 | |
Completeness [%] | 98.4 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 4000, magnesium acetate, ADA pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | ADA | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | magnesium acetate | ||
7 | 1 | 2 | ADA | ||
8 | 1 | 2 | H2O |