2FVM
Crystal structure of dihydropyrimidinase from Saccharomyces kluyveri in complex with the reaction product N-carbamyl-beta-alanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.843 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.141, 73.027, 164.105 |
Unit cell angles | 90.00, 91.98, 90.00 |
Refinement procedure
Resolution | 44.370 - 2.450 |
R-factor | 0.18477 |
Rwork | 0.182 |
R-free | 0.23743 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2fty |
RMSD bond length | 0.009 |
RMSD bond angle | 1.177 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.570 |
High resolution limit [Å] | 2.440 | 2.440 |
Number of reflections | 77328 | |
<I/σ(I)> | 12.1 | 4.8 |
Completeness [%] | 96.1 | 74 |
Redundancy | 3.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M trisodium citrate, 0.1M bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |