2FVM
Crystal structure of dihydropyrimidinase from Saccharomyces kluyveri in complex with the reaction product N-carbamyl-beta-alanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.843 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.141, 73.027, 164.105 |
| Unit cell angles | 90.00, 91.98, 90.00 |
Refinement procedure
| Resolution | 44.370 - 2.450 |
| R-factor | 0.18477 |
| Rwork | 0.182 |
| R-free | 0.23743 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2fty |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.570 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 77328 | |
| <I/σ(I)> | 12.1 | 4.8 |
| Completeness [%] | 96.1 | 74 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M trisodium citrate, 0.1M bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
