2FVL
Crystal structure of human 3-alpha hydroxysteroid/dihydrodiol dehydrogenase (AKR1C4) complexed with NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-23 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 166.008, 166.008, 194.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.730 - 2.400 |
| R-factor | 0.1672 |
| Rwork | 0.166 |
| R-free | 0.20454 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1XF0.pdb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 196.120 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 104763 | |
| Completeness [%] | 98.5 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Potassium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
