2FVH
Crystal Structure of Rv1848, a Urease Gamma Subunit UreA (Urea amidohydrolase), from Mycobacterium Tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 |
Unit cell lengths | 109.988, 109.988, 40.873 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.020 - 1.800 |
R-factor | 0.18625 |
Rwork | 0.184 |
R-free | 0.22946 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.602 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.043 | |
Number of reflections | 27463 | |
<I/σ(I)> | 28 | 2.2 |
Completeness [%] | 95.8 | 79.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 1.1% 2-Butanol, 23.76% Poly Ethylene Glycol 750MME, 0.16% Glycerol, 0.1M Sodium Cacodylate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |