2FUT
Crystal Structure of Heparinase II Complexed with a Disaccharide Product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.100, 163.300, 95.300 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 91.670 - 2.300 |
| R-factor | 0.17822 |
| Rwork | 0.176 |
| R-free | 0.22133 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.111 | 0.487 |
| Number of reflections | 126846 | |
| <I/σ(I)> | 10 | 2.05 |
| Completeness [%] | 94.5 | 84.9 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 20% PEG 3350, 0.2M magnesium chloride, pH 6.0, VAPOR DIFFUSION, temperature 298.0K |
