2FUF
Crystal structure of the SV40 large T antigen origin-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 |
| Unit cell lengths | 83.669, 83.669, 35.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.450 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.18900 |
| Structure solution method | SAD |
| Starting model (for MR) | 1tbd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.441 |
| Number of reflections | 24796 | 2416 |
| <I/σ(I)> | 53.6 | 4.5 |
| Completeness [%] | 98.7 | 96.3 |
| Redundancy | 10.9 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 1.6 M Sodium citrate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
