2FUB
Crystal structure of urate oxidase at 140 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.331 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.180, 95.630, 104.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.360 - 2.300 |
| R-factor | 0.16412 |
| Rwork | 0.161 |
| R-free | 0.21342 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.071 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.290 |
| Rmerge | 0.068 | 0.255 |
| Number of reflections | 17696 | |
| <I/σ(I)> | 9.2 | 2.6 |
| Completeness [%] | 97.8 | 95 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | PEG 3350(6-10%), 50-100mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
