2FTS

Crystal structure of the glycine receptor-gephyrin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	C 2 2 21
Unit cell lengths	51.298, 123.541, 155.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.410
Rwork	0.192
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.619
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.470
High resolution limit [Å]	2.380	5.130	2.380
Rmerge	0.096	0.049	0.563
Number of reflections	18221	2074	1488
<I/σ(I)>	10.6
Completeness [%]	89.6
Redundancy	4.8	4.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	311	0.1M Tris-HCl (pH 7.5), 0.1-0.2 M KSCN, 25-30% PEG 4000, vapor diffusion, hanging drop, temperature 311K