2FTA
Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM"
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.840, 97.630, 61.120 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.610 |
| Rwork | 0.233 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4azu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.350 |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.039 | 0.279 |
| Number of reflections | 55423 | 8203 |
| <I/σ(I)> | 18.01 | 4.4 |
| Completeness [%] | 99.4 | 0.987 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Potassium Thiocyanate, 30% PEG MME 2000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
