2FSD
A Common Fold for the Receptor Binding Domains of Lactococcal Phages? The Crystal Structure of the Head Domain of Phage bIL170
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93100 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.650, 69.650, 95.356 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.800 - 2.300 |
| R-factor | 0.24305 |
| Rwork | 0.242 |
| R-free | 0.26801 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.218 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.700 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 12327 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | nanodrop setting (300 nL), pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
