2FRZ
Crystal Structure of Cytochrome P450cam mutant (F87W/Y96F/V247L/C334A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.816, 62.114, 94.940 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.195 |
| Rwork | 0.190 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 1.200 |
| RMSD bond angle | 0.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 44876 | |
| Completeness [%] | 98.2 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M Sodium Acetate trihydrate, 0.1M Sodium Cacodylate, 25% PEG 8000, 0.01M spermidine , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
