2FRG

Structure of the immunoglobulin-like domain of human TLT-1

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-24
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	47.095, 79.981, 25.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.190
R-factor	0.17711
Rwork	0.176
R-free	0.19062
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Hybrid model containing structurally conserved residues of TREM-1 (1SMO chain A) NKp44(1HKF) and D1 domain of polymeric immunoglobulin receptor (1XED chain E).
RMSD bond length	0.010
RMSD bond angle	1.452
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.230
High resolution limit [Å]	1.190	1.190
Number of reflections	31487
<I/σ(I)>	25.9	3.2
Completeness [%]	98.2	87
Redundancy	6.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	Mother liquor contained 15-19% w/w PEG6000, 0.5M Na/K phosphate pH 5.6. Protein solution contained 10mg/ml TLT-1 in 20mM Tris pH 8. Protein and mother liquor were mixed in equal proportions at 15 C, and hanging drops were suspended over mother liquor., VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	Mother liquor contained 15-19% w/w PEG6000, 0.5M Na/K phosphate pH 5.6. Protein solution contained 10mg/ml TLT-1 in 20mM Tris pH 8. Protein and mother liquor were mixed in equal proportions at 15 C, and hanging drops were suspended over mother liquor., VAPOR DIFFUSION, HANGING DROP, temperature 293K