2FRE
The crystal structure of the oxidoreductase containing FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.337, 77.337, 151.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.840 - 1.900 |
| R-factor | 0.18407 |
| Rwork | 0.183 |
| R-free | 0.21240 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.268 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.840 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.134 | 0.800 |
| Number of reflections | 34404 | |
| <I/σ(I)> | 25.86 | 1.8 |
| Completeness [%] | 97.9 | 94.43 |
| Redundancy | 16.6 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.15M Potassium Bromide, 36% PEG2KMME, 10mM FMN, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
