2FR6
Crystal Structure of Mouse Cytidine Deaminase Complexed with Cytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.199, 92.961, 180.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.590 - 2.070 |
| Rwork | 0.188 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fr5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data scaling software | d*TREK (9.1SSI) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.590 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.079 | 0.345 |
| Total number of observations | 19190 | |
| Number of reflections | 42537 | 4184 |
| <I/σ(I)> | 11.4 | 3.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.6 | 4.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.9M AMMONIUM SULPHATE, 0.1M CITRATE, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
