2FQE
Crystal Structures of E. coli Laccase CueO under different copper binding situations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.587, 73.205, 184.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.060 - 1.920 |
| Rwork | 0.165 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 4.690 | 1.900 |
| Rmerge | 0.109 | 0.066 | 0.401 |
| Number of reflections | 35855 | 2550 | 2341 |
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 10% polyethylene glycol 4000, 15% Isopropanol, 100mM tri-Sodium Citrate dihydrate pH5.6, 0.5mM CuCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
