2FPU
Crystal Structure of the N-terminal domain of E.coli HisB- Complex with histidinol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.502, 132.664, 107.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.18548 |
| Rwork | 0.183 |
| R-free | 0.22296 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2fpr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 34717 | |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 96.8 | 81.7 |
| Redundancy | 4.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 25% (w/v) PEG 3350, 0.2M MgCl2, 0.1M Tris-HCl. , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
