2FPC
Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8057 |
| Spacegroup name | H 3 |
| Unit cell lengths | 148.607, 148.607, 121.069 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.1882 |
| Rwork | 0.186 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native structure |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.833 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.040 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.122 | 0.700 |
| Number of reflections | 19104 | |
| Completeness [%] | 96.0 | 95 |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | 0.8M potassium sodium tartrate tetrahydrate, 100mM HEPES-Na, 2mM secologanin, pH 7.5, VAPOR DIFFUSION, temperature 295.0K |
