2FP3

Crystal structure of the Drosophila initiator caspase Dronc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	I 41 3 2
Unit cell lengths	162.474, 162.474, 162.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.23
Rwork	0.222
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.055
Number of reflections	12899
Completeness [%]	99.7	100
Redundancy	26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	ammonium mono-hydrogen phosphate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	ammonium mono-hydrogen phosphate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K