2FNQ
Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality
Replaces: 1ZQ4Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-04-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.554, 78.023, 176.490 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 29.000 - 3.200 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 28928 | |
| Completeness [%] | 99.2 | 82.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG 8000, IMIDAZOLE ACETATE, SUCROSE, CALCIUM CHLORIDE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
